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Understanding otolith biomineralization processes: new insights into microscale spatial distribution of organic and mineral fractions from Raman microspectrometry ArchiMer
Jolivet, Aurelie; Bardeau, J.f.; Fablet, Ronan; Paulet, Y.m.; De Pontual, Helene.
It is generally accepted that the formation of otolith microstructures (L- and D-zones) and in particular the organic and mineral fractions vary on a daily basis. Raman microspectrometry provides a nondestructive technique that can be used to provide structural information on organic and mineral compounds. We applied it to thin otolith sections of hake in order to address the following issues: (1) the simultaneous characterization of variations in the organic and mineral fractions both in the core area and along successive otolith microstructures; (2) elucidation of significant differences between these fractions; (3) quantification of the effects of etching and staining protocols on otolith structures. The primordium appeared as a punctual area depicting...
Tipo: Text Palavras-chave: Acid etching; Staining; Aragonite; L and D zones; Core.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4355.pdf
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